Wound healing is a complex process and has been the subject of intense research for a long time. Plants are natural sources which have been widely used for wound healing. In current study, polarity based extracts of Syzygium cumini (L.), AzadirachtA.indica A. Juss, Eucalyptus globulus Labill and Nicotiana tabacum (L.) has been used for their wound healing potential on excision wounds after seven, fourteen and twenty one days in albino Wistar rats (both genders, 250-300 g). Statistically analyzed results (at p≤ 0.05) showed that among all plant, after day 07, day 14 and day 21, ethanolic extract from leaves of A.indica (A.) (37.5 %, 75 %, 95 % respectively) and E. glogulus (Labill.) (70 %, 95 % and 100 % respectively) and water extract of S.cumini (L.) (42.5 %, 87.5 % and 100 % respectively) and N. tabacum (L.) (7 %, 86 % and 91 % respectively) showed significant wound contraction in female rats as compared to male rats, while n-Hexane extract of all plants have least significant effects. Current study can be used as basic study for the isolation of active compound behind it so that it could be used for the formulation of curative bandage to cure excision wounds.