Background: Perilla frutescens (L.) Britton, a traditional herb belonging to the Lamiaceae family, is extensively cultivated in Manipur, a Northeastern State of India. Objective: This study aimed to investigate the proximate composition, essential oil content, and fatty acid composition of two cultivar variety of Perilla frutescens (L.) Britton. Methods: The proximate composition, including protein, total carbohydrates, and total fats, essential oil content, and fatty acid composition of Brown and white Perilla frutescens leaves and seeds were analyzed. Results: The results revealed that the leaves of White and Brown Perilla are rich in protein, total carbohydrates, and total fat content. The seeds contained significant amounts of essential oil, healthy fats, carbohydrates, and protein. Fatty acid composition analysis revealed high levels of alpha-linolenic acid (omega-6) and linolenic acid (omega3) in the seed. Conclusion: These findings suggest that Perilla frutescens (L.) Britton is an excellent source of essential nutrients, supporting its potential health benefits.