This comprehensive study aimed to evaluate the potential impacts of guar gum, on wound healing using a multi-faceted approach that included in silico, in vitro, and in vivo analyses. The in vitro experiments using the Vero cell line were conducted to evaluate the impact of guar gum treatment on cellular responses related to wound healing. The results revealed a significant increase in cell proliferation, as assessed by MTT assay, indicating the treatment's ability to promote cell growth. The crystal violet assay also demonstrated high cell viability in the guar gum-treated group, further supporting its positive effects on cellular activity. To evaluate the effects of guar gum therapy on wound healing in a physiological context, a rat burn model was used. The investigation showed that the treated group had a faster wound healing index than the control group, indicating a quicker healing time and better tissue regeneration. Furthermore, a significant decrease in Annexin V levels, a marker for apoptosis, was noticed in the treated group, suggesting a reduction in cell death and enhanced cell survival. These extensive findings from in vitro, and in vivo analyses collectively highlight the potential benefits of guar gum treatment in wound healing. The treatment exhibited favorable molecular interactions, promoted cell proliferation and viability in vitro, and facilitated faster wound healing with reduced apoptosis in vivo. While these results hold promise for the therapeutic application of guar gum in wound healing, further research is warranted to elucidate the underlying mechanisms and optimize treatment protocols. Additionally, larger clinical trials are necessary to evaluate the longterm safety and efficacy of guar gum treatment in human subjects. Finally, this study provides valuable insights into the potential of guar gum as a therapeutic intervention for enhancing wound healing outcomes, bridging the gap between, cellular responses, and physiological healing processes.